Measure the effects of changes in temperature, pH, and enzyme concentration on reaction rates of an enzyme catalyzed reaction in a controlled experiment. Explain how environmental factors affect the rate of enzyme-catalyzed reactions. INTRODUCTION: What would happen to your cells if they made a poisonous chemical? You might think that they would die. In fact, your cells are always making what is the enzyme for the catalase test chemicals.
They do not die because your cells use enzymes to break down these poisonous chemicals into harmless substances. Enzymes are proteins that speed up the rate of reactions that would otherwise happen more slowly. The enzyme is not altered by the reaction. You have hundreds of different enzymes in each of your cells. Each of these enzymes is responsible for one particular reaction that occurs in the cell.
In enzymes used in molecular cloning ppt lab, you will study an enzyme that is found in the cells of many living tissues. 2 harmless substances–water and oxygen. This reaction is important to cells because hydrogen peroxide is produced as a byproduct of many normal cellular reactions. If the cells did not break down the hydrogen peroxide, they would be poisoned and die. In this lab, you will study the catalase found in liver cells. You will be using chicken or beef liver.
It might seem strange to use dead cells to study the function of enzymes. This is possible because when normal levels of liver enzymes for dogs cell dies, the enzymes remain intact and active for several weeks, as long as the tissue is kept refrigerated. Using forceps and scissors cut a small piece of liver and add it to the test tube. Push it into the hydrogen peroxide with a stirring rod. What gas is being released? Feel the temperature of the test tube with your hand. Has it gotten warmer or colder?