Pearson, as an active contributor to the biology learning community, is pleased to provide free access to the Classic restriction enzyme digestion of dna lab activity of The Biology Place to all educators and their students. The purpose of the activities is to help you review material you have already studied in class or have read in your text. Some of the material will extend your knowledge beyond your classwork or textbook reading. At the end of each activity, you can assess your progress through a Self-Quiz. To begin, click on an activity title.
Concept 1: How Do Restriction Enzymes Work? Concept 11: Allelic What enzyme produced in the pancreas digests fat vs. Concept 3: How Do Guard Cells Function? Concept 5: The Genetic Code: RNA vs. Restriction enzymes are enzymes isolated from bacteria that recognize specific sequences in DNA and then cut the DNA to produce fragments, called restriction fragments. Restriction enzymes play a very important role in the construction of recombinant DNA molecules, as is done in gene cloning experiments. Another application of restriction enzymes is to map the locations of restriction sites in DNA.
You should have an understanding of DNA structure and the principles and steps involved in constructing and analyzing recombinant DNA molecules, as presented in lectures and in your textbook. This activity is designed to enhance your understanding and retention by illustrating DNA structure, restriction enzyme digestion of DNA, analysis of digested DNA by agarose gel electrophoresis, and the principles involved in constructing a restriction map from primary data. A 15-question multiple-choice quiz allows role of enzymes in biotechnology pdf to test your understanding of the material. An additional three questions test your ability to construct restriction maps from DNA fragment size data.